Kod: 06818914
This dissertation presents a novel technique of§delivering membrane impermeable molecules into single§living cells with the assistance of femtosecond (fs)§near-infrared (NIR) laser pulses. This technique§promises several major adv ... więcej
Za ten zakup dostaniesz 137 punkty
This dissertation presents a novel technique of§delivering membrane impermeable molecules into single§living cells with the assistance of femtosecond (fs)§near-infrared (NIR) laser pulses. This technique§promises several major advantages, namely, very high§transfection efficiency, high cell survival rate§( 100%) and fully preserved cell viabilities. §In this work, the system for fs NIR opto-injection§was designed and built. Successful fs NIR§opto-injection has been performed on several cell§systems including single mammalian cells, marine§animal eggs, and human cancer cells cultured in a§tissue-like environment. The connections between§laser parameters and cell responses were explored.§Dye uptake rate of the target cells was observed to§depend on incident laser intensity. Pore size was§found dependent on incident laser intensity. The§conclusion was made that laser-induced breakdown and§plasma-induced ablation in cell membrane are the§physical principles that govern the process of fs NIR§opto-injection.
Kategoria Książki po angielsku Mathematics & science Physics
54.64 €
Osobní odběr Bratislava a 2642 dalších
Copyright ©2008-24 najlacnejsie-knihy.sk Wszelkie prawa zastrzeżonePrywatnieCookies
Nákupní košík ( prázdný )