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Więcej informacji o Activation tagging in transgenic poplar

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Opis

We describe the establishment of an activation tagging system in poplar based upon the maize Ac/Ds transposable element system. Transgenic poplar plants were obtained carrying either one or two of the above mentioned constructs Following induction of the GIP promoter only a weak transposase signal could be detected in RT- PCR experiments. Mobility of the ATDs activation construct could not be confirmed in the GIP system. An Activation Tagging experiment was initiated using eight different double transgenic lines. A few putative variants could be obtained revealing deficiency in chlorophyll content. Single transgenic aspen lines carrying HSP- transposase have been produced. Following transformation with HSP-transposase a chimerical transgenic line was obtained revealing white and green plants. Following activation tagging experiment a HSP-transposase/ATDs-rolC double transgenic line, one somaclonal variation line was obtained revealing white plants.

Szczegóły książki

• Pełny tytuł: Activation tagging in transgenic poplar
• Podtytuł: Activation tagging in aspen using an inducible two component Ac/Ds-enhancer element system
• Autor: Fadia El Sherif, Hamdy Kamal, Matthias Fladung
• Język: Angielski
• Oprawa: Miękka
• Liczba stron: 120
• EAN: 9783639246674
• ID: 06832245
• Wydawca: VDM Verlag Dr. Müller
• Waga: 189 g
• Wymiary: 220 × 150 × 7 mm
• Rok wydania: 2010

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