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Book synopsis

This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.

Book details

Book category Books in English Mathematics & science Biology, life sciences Cellular biology (cytology)

• Full title: Surfaceome
• Subtitle: Methods and Protocols
• Author: Kenneth R. Boheler, Rebekah L. Gundry
• Language: English
• Binding: Hardback
• Number of pages: 348
• EAN: 9781493975518
• ISBN: 149397551X
• ID: 18184766
• Publisher: Humana Press Inc.
• Weight: 1155 g
• Dimensions: 254 × 178 × 26 mm
• Date of publishing: 20. December 2017

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