Code: 15996854

Molecular phylogenetic studies of pumpkin (Cucurbita argyrosperma) and winter melon (Benincasa hispida)

Name: Molecular phylogenetic studies of pumpkin (Cucurbita argyrosperma) and winter melon (Benincasa hispida)
Brand: Grin Publishing
SKU: 15996854
Price: 52.16 EUR
Availability: InStock

by Prem Jose Vazhacharickal, Sajeshkumar N. K, Jiby John Mathew, Anjana R, Dona Ann Johns

Language: English
Binding: Paperback
Number of pages: 32
Publisher: Grin Publishing, 2017
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More about Molecular phylogenetic studies of pumpkin (Cucurbita argyrosperma) and winter melon (Benincasa hispida)

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Scientific Study from the year 2017 in the subject Agrarian Studies, Mar Augusthinose College, language: English, abstract: The Cucurbitaceae family is the one of the economically important group of plants in the tropics and subtropics. Molecular phylogenetic analysis were advanced after the introduction of molecular markers which give much precise results in analysis. Our current study based on the amplification of RuBisco enzyme using rbcL primer and subsequent validation using BLAST, FASTA and CLUSTAL-W in pumpkin and winter melon. The isolated and purified DNA samples were PCR amplified using rbcL primer and later sequenced using ABI Prism 377 DNA sequencer. Multiple sequence alignment algorithms and distance matrix were constructed using rbcL sequences in FASTA format were retrieved from GenBank. Phylogenetic tree was created using the distance based neighbour joining (NJ) and clustering algorithms method. Once divergences between all pairs of samples were determined, statistical cluster analysis and dendrogams examines the similarity among halotypes. Bootstrapping and jackknifing further increase the reliability estimates for the position of haplotypes within the evolutionary tree.

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Book category Books in English Technology, engineering, agriculture Technology: general issues

52.16 €

Full title: Molecular phylogenetic studies of pumpkin (Cucurbita argyrosperma) and winter melon (Benincasa hispida)
Subtitle: Family comparison using rbcL sequence analysis
Author: Prem Jose Vazhacharickal, Sajeshkumar N. K, Jiby John Mathew, Anjana R, Dona Ann Johns
Language: English
Binding: Paperback
Number of pages: 32
EAN: 9783668383432
ISBN: 366838343X
ID: 15996854
Publisher: Grin Publishing
Weight: 121 g
Dimensions: 297 × 210 × 2 mm
Published: 2017