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Book synopsis

The ability of a cholesterol-reducing drug, simvastatin, to stimulate the formation of hydroxyapatite in an osteoblast-like cell line, was studied using Fourier transform infrared spectroscopy. Hydroxyapatite was detected using both Fourier transform infrared spectroscopy and von Kossa staining techniques a week earlier when cells were cultured in growth media supplemented with simvastatin in a tissue culture polystyrene system. Analysis of infrared spectra using area integration and curve-fitting techniques was performed to quantify the hydroxyapatite content in cells relative to the biological matrix. Infrared spectroscopy was used to quantify the mineralization process. Cells grown in a rotating bioreactor mineralized more rapidly than cells grown in tissue culture polystyrene systems.

Book details

Book category Books in English Technology, engineering, agriculture Industrial chemistry & manufacturing technologies Industrial chemistry

• Full title: Characterizing Osteoblast Mineralization Using Ftir
• Author: Alka Potdar
• Language: English
• Binding: Paperback
• Number of pages: 116
• EAN: 9783639253030
• ISBN: 3639253035
• ID: 06832786
• Publisher: VDM Verlag
• Weight: 181 g
• Dimensions: 229 × 152 × 7 mm
• Date of publishing: 20. May 2010

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